She was photographed by her long-time creative collaborator, Steven Meisel, in a setting reminiscent of a Paris café in the 1940s, wearing short skirts, ethnic-inspired sandals and animal-print bangles by the luxury goods firm.
The advertising campaign will break in the February 2009 issues of magazines worldwide and the first visuals show Madonna with the Kalahari bags in Monogram canvas and Monogram-embossed leather.
Antoine Arnault, head of communications for Louis Vuitton, said: "We are delighted to have Madonna as the star of our new fashion campaign.
"These are absolutely stunning shots whose rich imagery truly recaptures the magic of what was an unforgettable fashion show."